Relevance. The problem of listeriosis infection continues to be relevant in epizootology and epidemiology. Resistance to eradication of Listeria spp. This is due to the ability of the bacterium to adapt and persist in various environments, as well as its intracellular location and weak intracellular diffusion of certain antibiotics. Listeriosis refers to saprozoonoses, listeria often contaminates food products. As an animal pathogen, Listeria is of concern not only from the point of view of public health and food biosafety, but also as an animal pathogen leading to serious economic losses. The haphazard use of antibiotics to combat listeriosis has led to a change in the background of drug sensitivity.
The aim of the work is to determine the antibiotic resistance of current listeria strains and their sensitivity to bacteriophages in order to substantiate the prospects of phage treatments in the fight against listeriosis infection.
Methods. The methodology is based on routine bacteriological studies, spot tests and sensitivity determination using the disco diffusion method with 132 Listeria spp pathogens.
Results. Multiple antibiotic resistance to fosfomycin, meropenem, cefotaxime, bacitracin, tylosin, cephalexin, polymyxin-B, lincomycin, benzylpenicillin, cefpirom, cefaclor and other drugs was noted. Phagolysis of listeria by bacteriophages of our collection Lm1 (97.70%), Lm2 (96.20%) has been established. These bacteriophages are deposited in the collection of the Federal State Budgetary Scientific Institution "Federal Scientific Center — K.I. Scriabin and Ya.R. Kovalenko All-Russian Scientific Research Institute of Experimental Veterinary Medicine of the Russian Academy of Sciences". Thus, the aim of the study is to expand knowledge about listeriosis bacteriophages and phage use as an effective way to prevent, treat and control listeriosis infections in the food and livestock industry.
With the support of: The study was carried out within the framework of the state assignment of the Ministry of Science and Higher Education of the Russian Federation (project FGGG 2022-0007).
To quote:
Shastin P.N., Yakimova E.A., Supova A.V., Savinov V.A., Yezhova E.G., Khabarova A.V., Laishevtsev A.I. Antibiotic resistance and phage sensitivity of listeriosis pathogens. Agricultural science. 2024;(3):50-56. https://doi.org/10.32634/0869-8155-2024-380-3-50-56
The aim of the work is to determine the antibiotic resistance of current listeria strains and their sensitivity to bacteriophages in order to substantiate the prospects of phage treatments in the fight against listeriosis infection.
Methods. The methodology is based on routine bacteriological studies, spot tests and sensitivity determination using the disco diffusion method with 132 Listeria spp pathogens.
Results. Multiple antibiotic resistance to fosfomycin, meropenem, cefotaxime, bacitracin, tylosin, cephalexin, polymyxin-B, lincomycin, benzylpenicillin, cefpirom, cefaclor and other drugs was noted. Phagolysis of listeria by bacteriophages of our collection Lm1 (97.70%), Lm2 (96.20%) has been established. These bacteriophages are deposited in the collection of the Federal State Budgetary Scientific Institution "Federal Scientific Center — K.I. Scriabin and Ya.R. Kovalenko All-Russian Scientific Research Institute of Experimental Veterinary Medicine of the Russian Academy of Sciences". Thus, the aim of the study is to expand knowledge about listeriosis bacteriophages and phage use as an effective way to prevent, treat and control listeriosis infections in the food and livestock industry.
With the support of: The study was carried out within the framework of the state assignment of the Ministry of Science and Higher Education of the Russian Federation (project FGGG 2022-0007).
To quote:
Shastin P.N., Yakimova E.A., Supova A.V., Savinov V.A., Yezhova E.G., Khabarova A.V., Laishevtsev A.I. Antibiotic resistance and phage sensitivity of listeriosis pathogens. Agricultural science. 2024;(3):50-56. https://doi.org/10.32634/0869-8155-2024-380-3-50-56